Isolate and sequence ribosome-protected mRNA fragments using size-exclusion chromatography
نویسنده
چکیده
Investigating translationally active mRNA involves isolating and sequencing RNA directly associated with the ribosome. Traditionally, isolating these complexes involved sucrose gradient ultracentrifugation, which is tedious and technically difficult. ARTseqTM (active RNA translation) Ribosome Profiling Kits provide an alternative method for obtaining ribosome-protected RNA fragments using size-exclusion chromatography (SEC) columns. Both methods produce similar results, yet ARTseqTM offers a more rapid and simple method of enriching ribosome-bound mRNA fragments.
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The awesome power of ribosome profiling.
Ribosome profiling has proved to be a fantastic innovation which has revealed so much about mRNA translation that had previously gone unrecognized. The RNA community owes Jonathan Weissman and Nick Ingolia a deep sense of gratitude for conceiving the approach in the first place, and for their detailed optimization of the methodology. The origins of the approach can be traced back to the discove...
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